Susceptibility of HEK293 and RPTEC Cell Lines to Nephrotoxic Effects of Cefuroxime and Cefepime: A Comparative Study

Researchers need to identify the nephrotoxic properties of medicinal products both during preclinical development and when exploring options optimise pharmacotherapy. The main challenge is find an experimental model for assessing drug-induced nephrotoxicity that reflects in vivo conditions as closely possible. aim study was compare susceptibility HEK293 RPTEC cell lines used models cefuroxime cefepime. Materials methods. investigated cultured on plates with 0.4 µm pore membrane inserts. were incubated 3 days cefepime (cephalosporins excreted primarily by kidneys). added basal part well at concentrations 50 150 µg/mL (cefuroxime) or 30 120 (cefepime) twice a day. After incubating cells 24, 48, 72 hours, authors determined expression levels SLC22A6 SLC22A8 genes encoding organic anion transporters reverse transcription polymerase chain reaction. considered caspase 7 activation indicative effect cephalosporins; they evaluated this indicator fluorometric assay after hours incubation. Results. According study, decreased cephalosporin transport lines. decrease occurred line earlier than line. observed only incubation low (50 µg/mL, respectively) high (150 24 hours. Conclusions. exhibits higher toxic effects due transporter gene expression. Higher accelerate based promising tool analysis wide range products.